HSPG Antikörper (CF®740)

Produktnummer ABIN7654934

Der Ratte Monoklonal Anti-HSPG-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, HSPG in Proben von Human, Rind (Kuh), Maus, Fisch, Affe und Schwein zu detektieren.

Kurzübersicht für HSPG Antikörper (CF®740) (ABIN7654934)

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Reaktivität: Human, Rind (Kuh), Maus, Fisch, Affe, Schwein

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSPG Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: A7L6

Produktdetails anti-HSPG Antikörper (CF®740)

Verwendungszweck: Heparan Sulphate Proteoglycan(A7L6), CF740 conjugate

Produktmerkmale: This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan's strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Murine EHS laminin preparation

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 1-2 μg/mL. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Breast carcinomas, squamous cell carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu HSPG

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Andere Bezeichnung: Heparan Sulfate Proteoglycan

Hintergrund:

Synonyms: Heparan sulfate proteoglycan of basement membrane, HSPG, Hspg2, LG3 peptide, Perlecan, PLC, SJS1

Gene Symbol: HSPG2

Molekulargewicht: >400 kDa

Gen-ID: 3339

UniProt: P98160