Rekombinanter CD15 Antikörper (CF®740)

Produktnummer ABIN7654226

Der Kaninchen Monoklonal Anti-CD15-Antikörper wurde für FACS, IHC, IF und IHC (fp) validiert. Er ist geeignet, CD15 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Rekombinanter CD15 Antikörper (CF®740) (ABIN7654226)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD15 Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: FUT4-1478R

Produktdetails anti-CD15 Antikörper (CF®740)

Verwendungszweck: CD15 / FUT4 (Reed-Sternberg Cell Marker) (FUT4/1478R), CF740 conjugate

Produktmerkmale: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin's disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human neutrophils

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow cytometry 2-4 μg/million cells. Immunofluorescence 2-4 μg/mL. Immunohistology (formalin) 5-10 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: U937 cells, Reed-Sternberg cells in Hodgkin lymphoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Andere Bezeichnung: CD15

Hintergrund:

Synonyms: CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein ligand 1, PSGL1, Selectin P ligand, SELPLG

Gene Symbol: FUT4

Tissue Expression: Granulocytes

Molekulargewicht: 220 kDa

Gen-ID: 2526

UniProt: P22083