FTH1 Antikörper (AA 58-180)

Produktnummer ABIN7653997

Dieses Maus Monoklonal-Antikörper erkennt spezifisch FTH1 in WB, IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für FTH1 Antikörper (AA 58-180) (ABIN7653997)

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FTH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: FTH-2081

Produktdetails anti-FTH1 Antikörper

Bindungsspezifität: AA 58-180

Verwendungszweck: Ferritin, Heavy Chain (FTH) (Microglia Marker)(FTH/2081)

Produktmerkmale: Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of aging-related cataracts. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human FTH1 protein fragment (around aa 58-180)

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Andere Bezeichnung: Ferritin, Heavy Chain

Hintergrund:

Synonyms: Apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, Ferritin heavy chain, FHC, FRIH, FTH1, FTHL6, Iron overload autosomal dominant, PIG15, Placenta immunoregulatory factor, Proliferation inducing gene 15 protein (PLIF)

Gene Symbol: FTH1

Molekulargewicht: 21 kDa

Gen-ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis