FAT1 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN7653925

Dieses Maus Monoklonal-Antikörper erkennt spezifisch FAT1 in . Er zeigt eine Reaktivität gegenüber Drosophila melanogaster.

Kurzübersicht für FAT1 Antikörper (Cytoplasmic Domain) (ABIN7653925)

Target: FAT1 (FAT Tumor Suppressor Homolog 1 (FAT1))

Reaktivität: Drosophila melanogaster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FAT1 Antikörper ist unkonjugiert

Applikation: Bitte anfragen

Klon: FAT1-3D7-1

Produktdetails anti-FAT1 Antikörper

Bindungsspezifität: Cytoplasmic Domain

Verwendungszweck: FAT1 (FAT atypical cadherin 1)(FAT1-3D7/1)

Produktmerkmale: The FAT proteins are members of the Cadherin superfamily homologous to the Drosophila Fat protein that functions as a positive regulator of planar cell polarity in the Drosophila wing. FAT1 is an unusual cadherin that controls cell growth and planar polarity while acting as a tumor suppressor. FAT1 is a proximal element of a signaling pathway that determines both cellular polarity in the plane of the monolayer and directed actin-dependent cell motility. FAT1 is localized at the leading edge of lamellipodia, filopodia and microspike tips where it directly interacts with Ena/VASP proteins to regulate the actin polymerization complex. When targeted to mitochondrial outer leaflets, the cytoplasmic domain of FAT1 recruits components of the actin polymerization machinery sufficient to induce ectopic actin polymerization. FAT1 expression in vascular smooth muscle cells (VSMCs) increases significantly after arterial injury or growth factor stimulation, implicating FAT1 in the control of VSMC functions central to vascular remodeling by facilitating migration and limiting proliferation. FAT1 is also involved in psychiatric disorders, and its action may be of pathophysiological importance. This antibody is available purified, with BSA and azide (0.2 mg/mL) or purified, BSA- and azide-free (1 mg/mL).

Immunogen: Cytoplasmic domain of Drosophila Fat protein.

Isotyp: IgM, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: Wild type imaginal discs from third instar Drosophila larvae.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu FAT1

Target: FAT1 (FAT Tumor Suppressor Homolog 1 (FAT1))

Andere Bezeichnung: FAT1

Hintergrund:

Synonyms: Cadherin family member 7 precursor (CDHF7), Cadherin ME5, Cadherin related tumor suppressor homolog precursor (FAT protein homolog), FAT tumor suppressor homolog 1, hFat 1, Homolog of Drosophila tumor suppressor FAT precursor, nuclear form, Protein fat homolog, Protocadherin Fat 1

Gene Symbol: FAT1

Molekulargewicht: 500 kDa

Gen-ID: 2195

UniProt: Q14517