ERCC1 Antikörper (AA 191-281) (CF®568)

Produktnummer ABIN7653877

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ERCC1 in . Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ERCC1 Antikörper (AA 191-281) (CF®568) (ABIN7653877)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERCC1 Antikörper ist konjugiert mit CF®568

Applikation: Bitte anfragen

Klon: ERCC1-2683

Produktdetails anti-ERCC1 Antikörper (CF®568)

Bindungsspezifität: AA 191-281

Verwendungszweck: ERCC1 / RAD10 (Tumor Progression Marker)(ERCC1/2683), CF568 conjugate

Produktmerkmale: Recognizes a protein of 110 kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 191-281) of human ERCC1 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: HeLa, HepG2 cells. Tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Andere Bezeichnung: ERCC1

Hintergrund:

Synonyms: COFS4, DNA excision repair protein ERCC1, ERCC1, Excision repair cross complementing 1, RAD10, UV20

Gene Symbol: ERCC1

Molekulargewicht: ~110 kDa

Gen-ID: 2067

UniProt: P07992

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response