ENO2/NSE Antikörper (AA 416-433) (CF®405S)

Produktnummer ABIN7653500

Der Maus Monoklonal Anti-ENO2/NSE-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, ENO2/NSE in Proben von Human zu detektieren.

Kurzübersicht für ENO2/NSE Antikörper (AA 416-433) (CF®405S) (ABIN7653500)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ENO2/NSE Antikörper ist konjugiert mit CF®405S

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: NSE-P2

Produktdetails anti-ENO2/NSE Antikörper (CF®405S)

Bindungsspezifität: AA 416-433

Verwendungszweck: NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)(NSE-P2), CF405S conjugate

Produktmerkmale: This monoclonal antibody recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide of human NSE gamma (around aa416-433)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Andere Bezeichnung: NSE-Gamma

Hintergrund:

Synonyms: 2-phospho-D-glycerate hydrolyase, ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE

Gene Symbol: ENO2

Tissue Expression: Neurons

Molekulargewicht: ~50 kDa

Gen-ID: 2026

UniProt: P09104