Endoglin Antikörper (AA 74-251) (CF®740)

Produktnummer ABIN7653479

Der Maus Monoklonal Anti-Endoglin-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, Endoglin in Proben von Human zu detektieren.

Kurzübersicht für Endoglin Antikörper (AA 74-251) (CF®740) (ABIN7653479)

Target: Endoglin (ENG)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Endoglin Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: ENG-3269

Produktdetails anti-Endoglin Antikörper (CF®740)

Bindungsspezifität: AA 74-251

Verwendungszweck: Endoglin / CD105 (Angiogenesis Marker)(ENG/3269), CF740 conjugate

Produktmerkmale: CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for prostate adenocarcinoma, and cancers of the lung, stomach, breast, and brain. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Endoglin protein fragment (aa74-251)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa, A431 and HL-60 cells. Colon.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Endoglin

Target: Endoglin (ENG)

Andere Bezeichnung: CD105

Hintergrund:

Synonyms: CD105, END, Endoglin, Eng, HHT1, S-endoglin

Gene Symbol: ENG

Tissue Expression: Endothelial cells

Molekulargewicht: 84 kDa

Gen-ID: 2022

UniProt: P17813