Endoglin Antikörper (CF®740)

Produktnummer ABIN7653478

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Endoglin in FACS und IF. Er zeigt eine Reaktivität gegenüber Human, Ratte, Schwein, Pferd und Affe.

Kurzübersicht für Endoglin Antikörper (CF®740) (ABIN7653478)

Target: Endoglin (ENG)

Reaktivität: Human, Ratte, Schwein, Pferd, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Endoglin Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: ENG-1621

Produktdetails anti-Endoglin Antikörper (CF®740)

Verwendungszweck: Endoglin / CD105 (ENG/1621), CF740 conjugate

Produktmerkmale: CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for prostate adenocarcinoma, and cancers of the lung, stomach, breast, and brain. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant extracellular fragment of human Endoglin protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1-2 μg/million cells. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 2-4 μg/mL. ELISA: For coating order antibody without BSA. For functional studies, order Ab without azide. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa, A431 and HL-60 cells. Colon.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Endoglin

Target: Endoglin (ENG)

Andere Bezeichnung: CD105

Hintergrund:

Synonyms: CD105, END, Endoglin, Eng, HHT1, S-endoglin

Gene Symbol: ENG

Tissue Expression: Endothelial cells

Molekulargewicht: 84 kDa

Gen-ID: 2022

UniProt: P17813