Geminin Antikörper (CF®647)

Produktnummer ABIN7653260

Der Maus Monoklonal Anti-Geminin-Antikörper wurde für IF validiert. Er ist geeignet, Geminin in Proben von Human zu detektieren.

Kurzübersicht für Geminin Antikörper (CF®647) (ABIN7653260)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Geminin Antikörper ist konjugiert mit CF®647

Applikation: Immunofluorescence (IF)

Klon: CPTC-GMMN-1

Produktdetails anti-Geminin Antikörper (CF®647)

Verwendungszweck: Geminin / DNA Replication Inhibitor(CPTC-GMMN-1), CF647 conjugate

Produktmerkmale: Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphasepromoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitinmediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: HEK293, HeLa, MCF-7, Tonsil, Triple-negative breast cancer.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Andere Bezeichnung: DNA Replication Inhibitor

Hintergrund:

Synonyms: DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, GMMN, RP3 369A17.3

Gene Symbol: GMMN

Molekulargewicht: 35 kDa

Gen-ID: 51053

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication