Cytochrome C Antikörper (CF®740)

Produktnummer ABIN7652973

Dieses Anti-Cytochrome C-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cytochrome C in FACS und IF. Geeignet für Human, Ratte und Maus.

Kurzübersicht für Cytochrome C Antikörper (CF®740) (ABIN7652973)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 6H2-B4

Produktdetails anti-Cytochrome C Antikörper (CF®740)

Verwendungszweck: Cytochrome C (Mitochondrial Marker)(6H2.B4), CF740 conjugate

Produktmerkmale: Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Immunogen: Rat full-length cytochrome c protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: Cytochrome c

Hintergrund:

Synonyms: CYC, CYCS, HCS, THC4

Gene Symbol: CYCS

Molekulargewicht: 15 kDa

Gen-ID: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity