CD55 Antikörper (CF®740)

Produktnummer ABIN7652677

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD55 in FACS und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD55 Antikörper (CF®740) (ABIN7652677)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD55 Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 143-30

Produktdetails anti-CD55 Antikörper (CF®740)

Verwendungszweck: CD55 (DAF)(143-30), CF740 conjugate

Produktmerkmale: Recognizes a single chain glycoprotein of 70 kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: PHA stimulated human PBL

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (frozen) 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Jurkat, HUT-78, K562, YT, U937, MG63, and human lymphocytes. Human lymph nodes and tonsils.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Andere Bezeichnung: CD55

Hintergrund:

Synonyms: CD55|CD55 Cromer blood group system|Complement decay-accelerating factor|CROM|DAF|Daf-GPI|Decay Accelerating Factor for Complement|Decay accelerating factor GPI-form|Decay accelerating factor soluble-form|GPI-DAF

Gene Symbol: CD55

Molekulargewicht: 70 kDa

Gen-ID: 1604

UniProt: P08174

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity