GM-CSF Antikörper (CF®740)

Produktnummer ABIN7652488

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch GM-CSF in ELISA, FACS und IF. Er zeigt eine Reaktivität gegenüber Human, Cynomolgus und Rhesusaffen.

Kurzübersicht für GM-CSF Antikörper (CF®740) (ABIN7652488)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reaktivität: Human, Cynomolgus, Rhesusaffen

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser GM-CSF Antikörper ist konjugiert mit CF®740

Applikation: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: BVD2-21C11

Produktdetails anti-GM-CSF Antikörper (CF®740)

Verwendungszweck: GM-CSF(BVD2-21C11), CF740 conjugate

Produktmerkmale: Granulocyte/macrophage colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GM-CSF protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (frozen) 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Neutralization Studies order Ab without azide. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Lymph node and tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: GM-CSF

Hintergrund:

Synonyms: Burst Promoting Activity, Eosinophil Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sargramostim

Gene Symbol: CSF2

Molekulargewicht: 22 kDa

Gen-ID: 1437

UniProt: P04141

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin