Rekombinanter CD86 Antikörper (CF®740)

Produktnummer ABIN7652141

Der Maus Monoklonal Anti-CD86-Antikörper wurde für FACS, IHC, IF und IHC (fp) validiert. Er ist geeignet, CD86 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Rekombinanter CD86 Antikörper (CF®740) (ABIN7652141)

Target: CD86

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD86 Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: RC86-1146

Produktdetails anti-CD86 Antikörper (CF®740)

Verwendungszweck: CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF740 conjugate

Produktmerkmale: This antibody recognizes a protein of 70 kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CD86 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: 293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD86

Target: CD86

Andere Bezeichnung: CD86

Hintergrund:

Synonyms: Activation B7-2 antigen, B lymphocyte activation antigen B72, CD28 antigen ligand 2, CD28LG2, CLS1, CTLA-4 counter-receptor B7.2, Early T-cell co-stimulatory molecule 1, ETC-1, FUN-1, LAB72, Ly-58, MB7-2, TS/A-2

Gene Symbol: CD86

Tissue Expression: Dendritic cells|Monocytes/macrophages

Molekulargewicht: 70 kDa

Gen-ID: 942

UniProt: P42081

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation