CD80 Antikörper (AA 35-142) (CF®405S)

Produktnummer ABIN7652040

Dieses Anti-CD80-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD80 in . Geeignet für Human.

Kurzübersicht für CD80 Antikörper (AA 35-142) (CF®405S) (ABIN7652040)

Target: CD80

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD80 Antikörper ist konjugiert mit CF®405S

Applikation: Bitte anfragen

Klon: C80-2725

Produktdetails anti-CD80 Antikörper (CF®405S)

Bindungsspezifität: AA 35-142

Verwendungszweck: CD80 (B7-1)(C80/2725), CF405S conjugate

Produktmerkmale: T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31 % overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counter receptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human CD80 protein (around aa 35-142)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: Raji or Ramos Cells. Tonsil, Spleen or Thymus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD80

Target: CD80

Andere Bezeichnung: CD80

Hintergrund:

Synonyms: Activation B7-1 antigen, B lymphocyte activation antigen B7, B7, B7-1, BB1, CD28 antigen ligand 1, CD28LG, CD28LG1, CD80, Costimulatory factor CD80, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80

Gene Symbol: CD80

Tissue Expression: Antigen presenting cells

Molekulargewicht: 60 kDa

Gen-ID: 941

UniProt: P33681

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints