CD47 Antikörper (CF®740)

Produktnummer ABIN7651635

Dieses Anti-CD47-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD47 in FACS, IHC, IF und IHC (fp). Geeignet für Human und Maus.

Kurzübersicht für CD47 Antikörper (CF®740) (ABIN7651635)

Target: CD47

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD47 Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: IAP-964

Produktdetails anti-CD47 Antikörper (CF®740)

Verwendungszweck: CD47(IAP/964), CF740 conjugate

Produktmerkmale: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human IAP protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD47

Target: CD47

Andere Bezeichnung: CD47

Hintergrund:

Synonyms: Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen

Gene Symbol: CD47

Molekulargewicht: ~50 kDa

Gen-ID: 961

UniProt: Q08722