CD33 Antikörper (CF®740)

Produktnummer ABIN7651273

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD33 in FACS, IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD33 Antikörper (CF®740) (ABIN7651273)

Target: CD33

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD33 Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: WM53

Produktdetails anti-CD33 Antikörper (CF®740)

Verwendungszweck: CD33(WM53), CF740 conjugate

Produktmerkmale: Recognizes a 67 kDa glycoprotein, which is identified as CD33 (HLDA IV, WS Code M-505). CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immuno-receptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human AML cells

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL

Kommentare:

Positive Control: Human dendritic and mast cells. Human lymph nodes and tonsils.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD33

Target: CD33

Andere Bezeichnung: CD33

Hintergrund:

Synonyms: gp67, My9, Myeloid cell surface antigen CD33, p67, Sialic acid-binding Ig-like lectin 3 (SIGLEC3)

Gene Symbol: CD33

Tissue Expression: Dendritic cells|Monocytes/macrophages|Myeloid cells

Molekulargewicht: 67 kDa

Gen-ID: 945

UniProt: P20138