CDX2 Antikörper

Produktnummer ABIN7650758

Der Maus Monoklonal Anti-CDX2-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, CDX2 in Proben von Human zu detektieren.

Kurzübersicht für CDX2 Antikörper (ABIN7650758)

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDX2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: PCRP-CDX2-1A3

Produktdetails anti-CDX2 Antikörper

Verwendungszweck: CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) (PCRP-CDX2-1A3)

Produktmerkmale: The specificity of this monoclonal antibody to its intended target was validated by HuProt™Array, containing more than 19,000, full-length human proteins.The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CDX2protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Immunoprecipitation 2-4 μg/mg protein. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HT29 cells or Colon Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CDX2

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Andere Bezeichnung: CDX2

Hintergrund:

Synonyms: Caudal type homeobox 2, Caudal type homeobox transcription factor 2, Caudal-type homeobox protein 2,CDX2

Gene Symbol: CDX2

Tissue Expression: Intestine

Molekulargewicht: 40 kDa

Gen-ID: 1045

UniProt: Q99626

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance