Caldesmon Antikörper (High Molecular Weight)

Produktnummer ABIN7650419

Der Kaninchen Polyklonal Anti-Caldesmon-Antikörper wurde für WB, IHC und IHC (fp) validiert. Er ist geeignet, Caldesmon in Proben von Human und Ratte zu detektieren.

Kurzübersicht für Caldesmon Antikörper (High Molecular Weight) (ABIN7650419)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caldesmon Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-Caldesmon Antikörper

Bindungsspezifität: High Molecular Weight

Verwendungszweck: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)

Produktmerkmale: Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CALD1 protein fragment

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Andere Bezeichnung: Caldesmon

Hintergrund:

Synonyms: Caldesmon, Caldesmon 1, Caldesmon 1 Isoform 1, CDM, HCAD

Gene Symbol: CALD1

Tissue Expression: Smooth muscle

Molekulargewicht: 150 kDa

Gen-ID: 800

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction