Calcineurin B Antikörper (CF®647)

Produktnummer ABIN7650381

Dieses Anti-Calcineurin B-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Calcineurin B in . Geeignet für Human und Xenopus laevis.

Kurzübersicht für Calcineurin B Antikörper (CF®647) (ABIN7650381)

Target: Calcineurin B (CAN)

Reaktivität: Human, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Calcineurin B Antikörper ist konjugiert mit CF®647

Applikation: Bitte anfragen

Klon: BE2-1

Produktdetails anti-Calcineurin B Antikörper (CF®647)

Verwendungszweck: Calcineurin B / PPP3R1(BE2.1), CF647 conjugate

Produktmerkmale: This antibody recognizes the Calcineurin B alpha protein, encoded by the PPP3R1 (Protein Phosphatase 3 Regulatory Subunit B Alpha) gene. Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Calcineurin B protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: HeLa, MCF-7 or A431 cells. Human brain, breast or stomach tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Calcineurin B

Target: Calcineurin B (CAN)

Andere Bezeichnung: Calcineurin B

Hintergrund:

Synonyms: Calcineurin B, Calcineurin subunit B type 1, Protein Phosphatase 3 Regulatory Subunit B Alpha, CALNB1, Cna2, CNB, CNB1, PPP3R1

Gene Symbol: PPP3R1

Tissue Expression: Brain|T-cells

Molekulargewicht: 19 kDa

Gen-ID: 5534

UniProt: P63098

Pathways: Cellular Glucan Metabolic Process, VEGF Signaling