Rekombinanter beta-2 Microglobulin Antikörper (CF®647)

Produktnummer ABIN7650042

Dieses Anti-beta-2 Microglobulin-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von beta-2 Microglobulin in WB, FACS, IHC, IF und IHC (fp). Geeignet für Human und Non-Human Primate.

Kurzübersicht für Rekombinanter beta-2 Microglobulin Antikörper (CF®647) (ABIN7650042)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Non-Human Primate

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser beta-2 Microglobulin Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: B2M-1857R

Produktdetails anti-beta-2 Microglobulin Antikörper (CF®647)

Verwendungszweck: Beta-2 Microglobulin (Renal Failure & Tumor Marker) (B2M/1857R), CF647 conjugate

Produktmerkmale: Beta-2 microglobulin is a component of the class I major histocompatibility complex (MHC I) where it functions in antigen processing and presentation. Involved in the presentation of peptide antigens to the immune system. Human Beta-2 microglobulin is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This MAb is specific to human Beta-2 microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. The detection of Beta-2 microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human B2M protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Andere Bezeichnung: Beta-2 Microglobulin

Hintergrund:

Synonyms: B2M, Beta 2 microglobulin

Gene Symbol: B2M

Molekulargewicht: 12 kDa

Gen-ID: 567

UniProt: P61769

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process