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BCL2L1 Antikörper (AA 3-14) (CF®740)

Der Maus Monoklonal Anti-BCL2L1-Antikörper wurde für WB, IHC und IHC (fp) validiert. Er ist geeignet, BCL2L1 in Proben von Human, Maus, Ratte und Schwein zu detektieren.
Produktnummer ABIN7650014

Kurzübersicht für BCL2L1 Antikörper (AA 3-14) (CF®740) (ABIN7650014)

Target

Alle BCL2L1 Antikörper anzeigen
BCL2L1 (BCL2-Like 1 (BCL2L1))

Reaktivität

  • 236
  • 135
  • 119
  • 50
  • 25
  • 12
  • 10
  • 10
  • 5
  • 5
  • 4
  • 2
  • 2
  • 1
Human, Maus, Ratte, Schwein

Wirt

  • 170
  • 95
  • 1
Maus

Klonalität

  • 152
  • 114
Monoklonal

Konjugat

  • 139
  • 27
  • 11
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser BCL2L1 Antikörper ist konjugiert mit CF®740

Applikation

  • 180
  • 99
  • 65
  • 61
  • 57
  • 44
  • 32
  • 28
  • 28
  • 27
  • 21
  • 13
  • 10
  • 7
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon

BX006 2H12
  • Bindungsspezifität

    • 29
    • 25
    • 19
    • 16
    • 16
    • 15
    • 12
    • 10
    • 8
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 3-14

    Verwendungszweck

    bcl-x(BX006 + 2H12), CF740 conjugate

    Produktmerkmale

    Bcl-x belongs to the Bcl-2 family of proteins and plays a crucial role in apoptosis. Bcl-X has two isoforms: Bcl-XL (long), a 26 kDa protein which suppresses cell death, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2 and promotes apoptosis. This MAb reacts with both Bcl-XS and Bcl-XL proteins, and shows no cross-reaction with Bcl-2 or Bax proteins.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein (BX006 & 2H12)

    Isotyp

    IgG
  • Applikationshinweise

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

    Kommentare

    Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.1 mg/mL

    Buffer

    PBS, 0.1 % rBSA, 0.05 % azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Protect from light

    Lagerung

    4 °C

    Informationen zur Lagerung

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    Haltbarkeit

    24 months
  • Target

    BCL2L1 (BCL2-Like 1 (BCL2L1))

    Andere Bezeichnung

    Bcl-x

    Hintergrund

    Synonyms: Bcl-x, Bcl-2-like protein 1, BCL2L1, Apoptosis regulator Bcl-X

    Gene Symbol: BCL2L1

    Molekulargewicht

    27 kDa

    Gen-ID

    598

    UniProt

    Q07817

    Pathways

    Apoptose, Negative Regulation of intrinsic apoptotic Signaling
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