Aurora Kinase B Antikörper (AA 89-251) (CF®647)

Produktnummer ABIN7649750

Der Maus Monoklonal Anti-Aurora Kinase B-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, Aurora Kinase B in Proben von Human zu detektieren.

Kurzübersicht für Aurora Kinase B Antikörper (AA 89-251) (CF®647) (ABIN7649750)

Target: Aurora Kinase B (AURKB)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Aurora Kinase B Antikörper ist konjugiert mit CF®647

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: AURKB-1845

Produktdetails anti-Aurora Kinase B Antikörper (CF®647)

Bindungsspezifität: AA 89-251

Verwendungszweck: Aurora B (Proliferation Marker)(AURKB/1845), CF647 conjugate

Produktmerkmale: This antibody recognizes a protein of 39 kDa, which is identified as Aurora kinase B (or Aurora B). Aurora B is a serine/threonine protein kinase and component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. It is important for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 89-251) of human Aurora B protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: HeLa cells. Tonsil, Placenta, Hodgkin Lymphoma or Colon Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Andere Bezeichnung: Aurora kinase B

Hintergrund:

Synonyms: Aurora kinase B, Aurora B, Aurora-1, AURKB, AIK2, AIM-1, ARK-2, AurB, Aurora and Ipl1 like midbody associated protein 1, Aurora-B, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, IPL1, Protein phosphatase 1 regulatory subunit 48, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase aurora-B, STK1, STK12, STK5

Gene Symbol: AURKB

Molekulargewicht: 39 kDa

Gen-ID: 9212

UniProt: Q96GD4

Pathways: T-Zell Rezeptor Signalweg, Zellzyklus, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades