Liver Arginase Antikörper (AA 11-97) (Biotin)

Produktnummer ABIN7649677

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Liver Arginase in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Liver Arginase Antikörper (AA 11-97) (Biotin) (ABIN7649677)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Liver Arginase Antikörper ist konjugiert mit Biotin

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: ARG1-1126

Produktdetails anti-Liver Arginase Antikörper (Biotin)

Bindungsspezifität: AA 11-97

Verwendungszweck: Arginase 1 (Hepatocellular Carcinoma Marker) (ARG1/1126) , Biotin conjugate

Produktmerkmale: This antibody recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibodies to Arginase 1 label hepatocytes in normal tissues and granulocytes in peripheral blood. Arginase 1 is a sensitive and specific marker for identification of hepatocellular carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ARG1 protein fragment (around aa11-97)

Isotyp: IgG3, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: 293Tcells. Hepatocellular Carcinoma (HCC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Andere Bezeichnung: Arginase 1

Hintergrund:

Synonyms: Arginase 1, ARG1, liver-type arginase, type I arginase

Gene Symbol: ARG1

Tissue Expression: Liver

Molekulargewicht: 35-38 kDa

Gen-ID: 383

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin