EIF4E Antikörper (pSer209)

Produktnummer ABIN7603287

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch EIF4E in WB, IHC, FACS und IP. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für EIF4E Antikörper (pSer209) (ABIN7603287)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser EIF4E Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon: AFHI-5

Produktdetails anti-EIF4E Antikörper

Bindungsspezifität: pSer209

Verwendungszweck: Anti-EIF4E Monoclonal Antibody

Produktmerkmale: Anti-EIF4E Monoclonal Antibody (ABIN7603287). Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human EIF4E eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:100-1:5000
IHC 1:100-1:500
IP 1:30
FC 1:40

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Konzentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Details zu EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Andere Bezeichnung: EIF4E

Hintergrund:

Synonyms: Transcriptional repressor CTCF,11-zinc finger protein,CCCTC-binding factor,CTCFL paralog,CTCF,

Tissue Specificity: Ubiquitous. Absent in primary spermatocytes. .

Molekulargewicht: 25 kDa

UniProt: P06730

Pathways: BCR Signaling