GABARAP Antikörper (N-Term)

Produktnummer ABIN7603159

Der Kaninchen Polyklonal Anti-GABARAP-Antikörper wurde für WB, IHC und FACS validiert. Er ist geeignet, GABARAP in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für GABARAP Antikörper (N-Term) (ABIN7603159)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABARAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-GABARAP Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-GABARAP Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-GABARAP Antibody Picoband® (ABIN7603159). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GABARAP, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
1. Behrends, C., Sowa, M. E., Gygi, S. P., Harper, J. W. Network organization of the human autophagy system. Nature 466: 68-76, 2010 2. Hemelaar, J., Lelyveld, V. S., Kessler, B. M., Ploegh, H. L. A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L. J. Biol. Chem. 278: 51841-51850, 2003. 3. Komoike, Y., Shimojima, K., Liang, J.-S., Fujii, H., Maegaki, Y., Osawa, M., Fujii, S., Higashinakagawa, T., Yamamoto, T. A functional analysis of GABARAP on 17p13.1 by knockdown zebrafish. J. Hum. Genet. 55: 155-162, 2010.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Andere Bezeichnung: GABARAP

Hintergrund:

Synonyms: C-C chemokine receptor type 1,C-C CKR-1,CC-CKR-1,CCR-1,CCR1,Macrophage inflammatory protein 1-alpha receptor,MIP-1alpha-R,RANTES-R,CD191,Ccr1,Cmkbr1,

Tissue Specificity: Detected in the heart, spleen, lung, peritoneal exudate cells and leukocytes.

Background: Gamma-aminobutyric acid receptor-associated protein is a protein that in humans is encoded by the GABARAP gene. Gamma-aminobutyric acid A receptors [GABA (A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA (A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

Molekulargewicht: 18 kDa

Gen-ID: 11337

UniProt: O95166

Pathways: Autophagie