Vinexin Antikörper (N-Term)

Produktnummer ABIN7603121

Dieses Anti-Vinexin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Vinexin in WB, IHC, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Vinexin Antikörper (N-Term) (ABIN7603121)

Target: Vinexin (Sorbs3) (Sorbin and SH3 Domain Containing 3 (Sorbs3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Vinexin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-Vinexin Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-Vinexin/SORBS3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Vinexin/SORBS3 Antibody Picoband® (ABIN7603121). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Vinexin/SORBS3, which shares 87.5% and 84.4% amino acid (aa) sequence identity with mouse and rat Vinexin/SORBS3, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Kioka, N., Sakata, S., Kawauchi, T., Amachi, T., Akiyama, S. K., Okazaki, K., Yaen, C., Yamada, K. M., Aota, S. Vinexin: a novel vinculin-binding protein with multiple SH3 domains enhances actin cytoskeletal organization. J. Cell Biol. 144: 59-69, 1999. 2. Mitsushima, M., Ueda, K., Kioka, N. Vinexin-beta regulates the phosphorylation of epidermal growth factor receptor on the cell surface. Genes Cells 11: 971-982, 2006. 3. Suwa, A., Mitsushima, M., Ito, T., Akamatsu, M., Ueda, K., Amachi, T., Kioka, N. Vinexin-beta regulates the anchorage dependence of ERK2 activation stimulated by epidermal growth factor. J. Biol. Chem. 277: 13053-13058, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Vinexin

Target: Vinexin (Sorbs3) (Sorbin and SH3 Domain Containing 3 (Sorbs3))

Andere Bezeichnung: SORBS3

Hintergrund:

Synonyms: Vinexin, SH3-containing adapter molecule 1, SCAM-1, Sorbin and SH3 domain-containing protein 3, SORBS3, SCAM1

Tissue Specificity: Both isoforms are expressed in different tissues like heart, placenta, brain, skeletal muscle and pancreas. Isoform beta is especially found in liver.

Background: Vinexin is a protein that in humans is encoded by the SORBS3 gene. It is mapped to 8p21.3. This gene encodes an SH3 domain-containing adaptor protein. The presence of SH3 domains play a role in this protein's ability to bind other cytoplasmic molecules and contribute to cystoskeletal organization, cell adhesion and migration, signaling, and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 87 kDa

Gen-ID: 10174

UniProt: O60504

Pathways: MAPK Signalweg