GAPDH Antikörper (C-Term) (HRP)

Produktnummer ABIN7602946

Der Kaninchen Polyklonal Anti-GAPDH-Antikörper wurde für WB validiert. Er ist geeignet, GAPDH in Proben von Human, Maus, Ratte, Huhn, Affe und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für GAPDH Antikörper (C-Term) (HRP) (ABIN7602946)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB)

Produktdetails anti-GAPDH Antikörper (HRP)

Bindungsspezifität: C-Term

Verwendungszweck: Anti-GAPDH Antibody Picoband® (HRP)

Kreuzreaktivität: Huhn, Human, Affe, Maus, Ratte, Zebrafisch (Danio rerio)

Kreuzreaktivität (Details): HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.

Produktmerkmale: Anti-GAPDH Antibody (HRP) (-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: HRP-GAPDH antibody is affinity chromatography purified via peptide column.

Immunogen: HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.02-0.1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 0.5 mg/mL

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: HRP-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund:

Synonyms: HRP-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD

Tissue Specificity: Widely expressed, with the highest expression in lung, spleen and thymus.

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Sequence Similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

Molekulargewicht: 36 kDa

UniProt: P04406