Nibrin Antikörper (C-Term)

Produktnummer ABIN7602793

Der Maus Monoklonal Anti-Nibrin-Antikörper wurde für WB, IHC, IF, ICC und FACS validiert. Er ist geeignet, Nibrin in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Nibrin Antikörper (C-Term) (ABIN7602793)

Target: Nibrin (NBN)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nibrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 5D7A1

Produktdetails anti-Nibrin Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-p95 NBS1 Antibody Picoband® (monoclonal, 5D7A1)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-p95 NBS1 Antibody Picoband® (monoclonal, 5D7A1) (ABIN7602793). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human p95 NBS1, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human
1. "Atlas of Genetics and Cytogenetics in Oncology and Haematology - NBS1". 2. Carney JP, Maser RS, Olivares H, Davis EM, Le Beau M, Yates JR, Hays L, Morgan WF, Petrini JH (May 1998). "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response". Cell 93 (3): 477-86. 3. Varon R, Vissinga C, Platzer M, Cerosaletti KM, Chrzanowska KH, Saar K, Beckmann G, Seemanová E, Cooper PR, Nowak NJ, Stumm M, Weemaes CM, Gatti RA, Wilson RK, Digweed M, Rosenthal A, Sperling K, Concannon P, Reis A (May 1998). "Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome". Cell 93 (3): 467-76.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu Nibrin

Target: Nibrin (NBN)

Andere Bezeichnung: NBN

Hintergrund:

Synonyms: Apoptic death agonist antibody, Apoptotic death agonist antibody, Apoptotic death agonist BID antibody, BH3 interacting domain death agonist antibody, BH3 interacting domain death agonist p11 antibody, BH3 interacting domain death agonist p13 antibody, BH3 interacting domain death agonist p15 antibody, BH3-interacting domain death agonist p11 antibody, BID antibody, BID isoform ES (1b) antibody, BID isoform L (2) antibody, BID isoform Si6 antibody, BID_HUMAN antibody, Desmocollin type 4 antibody, FP497 antibody, Human BID coding sequence antibody, MGC15319 antibody, MGC42355 antibody, p11 BID antibody, p13 BID antibody, p15 BID antibody, p22 BID antibody

Tissue Specificity: Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).

Background: p95 NBS1, also known as NBN or Nibrin, is a protein which in humans is encoded by the NBN gene. Nibrin is a protein associated with the repair of double strand breaks (DSBs) which pose serious damage to a genome. It is a 754 amino acid protein identified as a member of the NBS1/hMre11/RAD50(N/M/R, more commonly referred to asMRN) double strand DNA break repair complex. This complex recognizes DNA damage and rapidly relocates to DSB sites and forms nuclear foci. It also has a role in regulation of N/M/R (MRN) protein complex activity which includes end-processing of both physiological and mutagenic DNA double strand breaks (DSBs).

Molekulargewicht: 95 kDa

Gen-ID: 4683

UniProt: O60934

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response