NT5DC2 Antikörper (AA 9-520)

Produktnummer ABIN7602634

Produktdetails anti-NT5DC2 Antikörper

Target: NT5DC2 (5'-Nucleotidase Domain Containing 2 (NT5DC2))

Bindungsspezifität: AA 9-520

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NT5DC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Verwendungszweck: Anti-NT5DC2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NT5DC2 Antibody Picoband® (ABIN7602634). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NT5DC2 recombinant protein (Position: Q9-R520).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Zhu, Z. , Hou, Q. , & Guo, H. . (2020). Nt5dc2 knockdown inhibits colorectal carcinoma progression by repressing metastasis, angiogenesis and tumor-associated macrophage recruitment: a mechanism involving vegf signaling. Experimental Cell Research, 397( 1). 2. A, X. J. , B, X. L. , C, Z. Z. , & A, L. X. . (2020). Nt5dc2 suppression restrains progression towards metastasis of non-small-cell lung cancer through regulation p53 signaling. Biochemical and Biophysical Research Communications, 533( 3), 354-361. 3. Li, K. S. , Zhu, X. D. , Liu, H. D. , Zhang, S. Z. , & Sun, H. C. . (2020). Nt5dc2 promotes tumor cell proliferation by stabilizing egfr in hepatocellular carcinoma. Cell Death & Disease, 11(5).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NT5DC2

Target: NT5DC2 (5'-Nucleotidase Domain Containing 2 (NT5DC2))

Andere Bezeichnung: NT5DC2 (NT5DC2 Produkte)

Hintergrund:

Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: 5'-Nucleotidase domain containing 2 (NT5DC2) is a member of the NT5DC family and contains a haloacid dehalogenase motif localized in the N-terminus of these proteins. NT5DC2 shows high sequence similarity with NT5C2. NT5C2 has received attention in the field of hematological neoplasms because NT5C2 mutations have been demonstrated to drive resistance to thiopurine, a drug frequently used to treat hematological neoplasms. NT5C2 catalyzes purine-nucleotide metabolism and induces chemotherapeutic resistance via excess export of purines to the extracellular space and depletion of the intracellular purine-nucleotide pool. Therefore, NT5DC2 may also participate in purine-nucleotide metabolism and exert purine-nucleotide catalytic activity. NT5DC2 overexpression promotes HCC cell proliferation and clone formation by regulating the cell cycle and promoting tumor growth in subcutaneous xenografts, while NT5DC2 knockdown inhibits these processes. NT5DC2 is associated with attention-deficit/hyperactivity disorder and bipolar disorder. NT5DC2 interacts with tyrosine hydroxylase (TH) to regulate TH catalytic activity and thus regulate catecholamine synthesis. NT5DC2 has been shown to interact with and stabilize Fyn, an Src family proto-oncogene, and plays a role in regulating glioblastoma progression.

Molekulargewicht: 61 kDa

Gen-ID: 64943

UniProt: Q9H857