NLRP3 Antikörper (AA 812-1035)

Produktnummer ABIN7602532

Der Kaninchen Polyklonal Anti-NLRP3 Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, NLRP3 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für NLRP3 Antikörper (AA 812-1035) (ABIN7602532)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NLRP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-NLRP3 Antikörper

Bindungsspezifität: AA 812-1035

Verwendungszweck: Anti-CIAS1/NALP3/NLRP3 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CIAS1/NALP3/NLRP3 Antibody (ABIN7602532). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CIAS1/NALP3/NLRP3 recombinant protein (Position: D812-S1035).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NLRP3

Hintergrund:

Synonyms: Neurogenic locus notch homolog protein 1,Notch 1,hN1,Translocation-associated notch protein TAN-1,Notch 1 extracellular truncation,NEXT,Notch 1 intracellular domain,NICD,NOTCH1,TAN1,

Tissue Specificity: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

Background: NLRP3 (NLR FAMILY, PYRIN DOMAIN-CONTAINING 3), also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 (NOD-like receptor family, pryin domain containing 3) gene. The NLRP3 gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. And the NLRP3 gene is mapped on 1q44. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD (ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, Gross et al.showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

Molekulargewicht: 118 kDa

Gen-ID: 114548

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome