KPNB1 Antikörper (AA 8-876)

Produktnummer ABIN7602501

Dieses Maus Monoklonal-Antikörper erkennt spezifisch KPNB1 in WB, IHC, FACS, IF und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für KPNB1 Antikörper (AA 8-876) (ABIN7602501)

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KPNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 3I11F2

Produktdetails anti-KPNB1 Antikörper

Bindungsspezifität: AA 8-876

Verwendungszweck: Anti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) (ABIN7602501). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human KPNB1 recombinant protein (Position: E8-A876).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Ayala-Madrigal, M. L., Doerr, S., Ramirez-Duenas, M. L., Hansmann, I. Assignment of KPNA4 and KPNB1 encoding karyopherin alpha 4 and beta 1 to human chromosome bands 11q22 and 17q21 respectively, by in situ hybridization. Cytogenet. Cell Genet. 89: 258-259, 2000. 2. Bayliss, R., Littlewood, T., Stewart, M. Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking. Cell 102: 99-108, 2000. 3. Caudron, M., Bunt, G., Bastiaens, P., Karsenti, E. Spatial coordination of spindle assembly by chromosome-mediated signaling gradients. Science 309: 1373-1376, 2005.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu KPNB1

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Andere Bezeichnung: KPNB1

Hintergrund:

Synonyms: Peroxiredoxin-6,1.11.1.15,1-Cys peroxiredoxin,1-Cys PRX,24 kDa protein,Acidic calcium-independent phospholipase A2,aiPLA2,3.1.1.-,Antioxidant protein 2,Liver 2D page spot 40,Non-selenium glutathione peroxidase,NSGPx,1.11.1.9,Red blood cells page spot 12,PRDX6,AOP2, KIAA0106,

Tissue Specificity: Widely expressed in fetal and adult tissues.

Background: Importin subunit beta-1 is a protein that in humans is encoded by the KPNB1 gene. Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 97 kDa

Gen-ID: 3837

UniProt: Q14974

Pathways: Protein targeting to Nucleus