PREX1 Antikörper (AA 643-1155)

Produktnummer ABIN7602223

Der Kaninchen Polyklonal Anti-PREX1-Antikörper wurde für ELISA, WB und FACS validiert. Er ist geeignet, PREX1 in Proben von Human zu detektieren.

Kurzübersicht für PREX1 Antikörper (AA 643-1155) (ABIN7602223)

Target: PREX1 (Phosphatidylinositol-3,4,5-Trisphosphate-Dependent Rac Exchange Factor 1 (PREX1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PREX1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-PREX1 Antikörper

Bindungsspezifität: AA 643-1155

Verwendungszweck: Anti-PREX1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PREX1 Antibody Picoband® (ABIN7602223). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PREX1 recombinant protein (Position: N643-D1155).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Donald, S., Humby, T., Fyfe, I., Segonds-Pichon, A., Walker, S. A., Andrews, S. R., Coadwell, W. J., Emson, P., Wilkinson, L. S., Welch, H. C. E. P-Rex2 regulates Purkinje cell dendrite morphology and motor coordination. Proc. Nat. Acad. Sci. 105: 4483-4488, 2008. 2. Welch, H. C. E., Coadwell, W. J., Ellson, C. D., Ferguson, G. J., Andrews, S. R., Erdjument-Bromage, H., Tempst, P., Hawkins, P. T., Stephens, L. R. P-Rex1, a PtdIns(3,4,5)P3- and G-beta-gamma-regulated guanine-nucleotide exchange factor for Rac. Cell 108: 809-821, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PREX1

Target: PREX1 (Phosphatidylinositol-3,4,5-Trisphosphate-Dependent Rac Exchange Factor 1 (PREX1))

Andere Bezeichnung: PREX1

Hintergrund:

Synonyms: NADPH oxidase 5, NOX5

Tissue Specificity: Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level).

Background: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein is a protein that in humans is encoded by the PREX1 gene. The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.

Molekulargewicht: 186 kDa

Gen-ID: 57580

Pathways: Regulation of Actin Filament Polymerization