B-Cell Linker Antikörper (AA 59-456)

Produktnummer ABIN7602100

Der Kaninchen Polyklonal Anti-B-Cell Linker-Antikörper wurde für WB, ELISA, IF und ICC validiert. Er ist geeignet, B-Cell Linker in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für B-Cell Linker Antikörper (AA 59-456) (ABIN7602100)

Target: B-Cell Linker (BLNK)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser B-Cell Linker Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-B-Cell Linker Antikörper

Bindungsspezifität: AA 59-456

Verwendungszweck: Anti-BLNK Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-BLNK Antibody Picoband® (ABIN7602100). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human BLNK recombinant protein (Position: D59-S456).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Flemming, A., Brummer, T., Reth, M., Jumaa, H. The adaptor protein SLP-65 acts as a tumor suppressor that limits pre-B cell expansion. Nature Immun. 4: 38-43, 2003. 2. Fu, C., Turck, C. W., Kurosaki, T., Chan, A. C. BLNK: a central linker protein in B cell activation. Immunity 9: 93-103, 1998. 3. Hayashi, K., Nojima, T., Goitsuka, R., Kitamura, D. Impaired receptor editing in the primary B cell repertoire of BASH-deficient mice. J. Immun. 173: 5980-5988, 2004.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu B-Cell Linker

Target: B-Cell Linker (BLNK)

Andere Bezeichnung: BLNK

Hintergrund:

Synonyms: Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, LMAN1, ERGIC53, F5F8D

Tissue Specificity: Ubiquitous.

Background: The B-cell linker protein is encoded by the BLNK gene and is an adaptor protein also known as SLP-65, BASH, and BCA. This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene.

Molekulargewicht: 70 kDa

Gen-ID: 29760

Pathways: BCR Signaling