PIWIL4 Antikörper (AA 51-749)

Produktnummer ABIN7601940

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PIWIL4 in WB, ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PIWIL4 Antikörper (AA 51-749) (ABIN7601940)

Target: PIWIL4 (Piwi-Like 4 (PIWIL4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIWIL4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-PIWIL4 Antikörper

Bindungsspezifität: AA 51-749

Verwendungszweck: Anti-PIWIL4/PIWI Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PIWIL4/PIWI Antibody Picoband® (ABIN7601940). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PIWIL4/PIWI recombinant protein (Position: R51-N749).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Carmell, M. A., Girard, A., van de Kant, H. J. G., Bourc'his, D., Bestor, T. H., de Rooij, D. G., Hannon, G. J. MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev. Cell 12: 503-514, 2007. 2. De Fazio, S., Bartonicek, N., Di Giacomo, M., Abreu-Goodger, C., Sankar, A., Funaya, C., Antony, C., Moreira, P. N., Enright, A. J., O'Carroll, D. The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements. Nature 480: 259-263, 2011. 3. Sasaki, T., Shiohama, A., Minoshima, S., Shimizu, N. Identification of eight members of the Argonaute family in the human genome. Genomics 82: 323-330, 2003.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PIWIL4

Target: PIWIL4 (Piwi-Like 4 (PIWIL4))

Andere Bezeichnung: PIWIL4

Hintergrund:

Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2

Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.

Background: PIWI-like protein 4 (PIWIL4) is a 97 kDa member of the argonaute family of proteins and the PIWI subfamily. Human PIWIL4 is 852 amino acids (aa) in length and contains one PAZ domain (aa 268-384) and one PIWI domain (aa 546-838). There are three isoforms for human PIWIL4. Isoform 1 is the standard protein. Isoform 2 has a two aa substitution for aa 1-29 in isoform 1 and a deletion of aa 523-852. Isoform 3 has a deletion of aa 1-69 and a deletion of aa 523-852. Human PIWIL4 shares 77 % aa sequence identity with mouse and rat PIWIL4. PIWIL4 plays a central role in spermatogenesis.

Molekulargewicht: 97 kDa

Gen-ID: 143689

UniProt: Q7Z3Z4