PTRF Antikörper (AA 47-390)

Produktnummer ABIN7601817

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PTRF in WB, ELISA, IHC, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PTRF Antikörper (AA 47-390) (ABIN7601817)

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTRF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-PTRF Antikörper

Bindungsspezifität: AA 47-390

Verwendungszweck: Anti-PTRF/CAVIN1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PTRF/CAVIN1 Antibody Picoband® (ABIN7601817). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PTRF/CAVIN1 recombinant protein (Position: D47-D390).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hayashi, Y. K., Matsuda, C., Ogawa, M., Goto, K., Tominaga, K., Mitsuhashi, S., Park, Y.-E., Nonaka, I., Hino-Fukuyo, N., Haginoya, K., Sugano, H., Nishino, I. Human PTRF mutations cause secondary deficiency of caveolins resulting in muscular dystrophy with generalized lipodystrophy. J. Clin. Invest. 119: 2623-2633, 2009. 2. Hill, M. M., Bastiani, M., Luetterforst, R., Kirkham, M., Kirkham, A., Nixon, S. J., Walser, P., Abankawa, D., Oorschot, V. M. J., Martin, S., Hancock, J. F., Parton, R. G. PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function. Cell 132: 113-124, 2008. 3. Jansa, P., Mason, S. W., Hoffmann-Rohrer, U., Grummt, I. Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes. EMBO J. 17: 2855-2864, 1998.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PTRF

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Andere Bezeichnung: CAVIN1

Hintergrund:

Synonyms: Lymphocyte antigen 6A-2/6E-1,Ly-6A.2/Ly-6E.1,Stem cell antigen 1,SCA-1,T-cell-activating protein,TAP,Ly6a,Ly6,

Tissue Specificity: Widely expressed. .

Background: This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.

Molekulargewicht: 50-55 kDa

Gen-ID: 284119