Western Blotting (WB), ELISA, Flow Cytometry (FACS)
Verwendungszweck
Anti-POLE2 Antibody Picoband®
Produktmerkmale
Anti-POLE2 Antibody Picoband® (ABIN7601802). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Aufreinigung
Immunogen affinity purified.
Immunogen
E.coli-derived human POLE2 recombinant protein (Position: E46-K483). Human POLE2 shares 99.1% amino acid (aa) sequence identity with mouse POLE2.
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL, - 1. Frugoni, F., Dobbs, K., Felgentreff, K., Aldhekri, H., Al Saud, B. K., Arnaout, R., Ali, A. A., Abhyankar, A., Alroqi, F., Giliani, S., Ojeda, M. M., Tsitsikov, E., Pai, S.-Y., Casanova, J. L., Notarangelo, L. D., Manis, J. P. A novel mutation in the POLE2 gene causing combined immunodeficiency. J. Allergy Clin. Immun. 137: 635-638, 2016. 2. Li, Y., Asahara, H., Patel, V. S., Zhou, S., Linn, S. Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon. J. Biol. Chem. 272: 32337-32344, 1997. 3. Stumpf, A. M. Personal Communication. Baltimore, Md. 03/26/2021.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
DNA polymerase epsilon subunit 2 is an enzyme that in humans is encoded by the POLE2 gene. DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency.