NNT Antikörper (AA 44-350)

Produktnummer ABIN7601742

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NNT in WB, ELISA, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schwein und Affe.

Kurzübersicht für NNT Antikörper (AA 44-350) (ABIN7601742)

Target: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Reaktivität: Human, Maus, Ratte, Schwein, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NNT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NNT Antikörper

Bindungsspezifität: AA 44-350

Verwendungszweck: Anti-NNT Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NNT Antibody Picoband® (ABIN7601742). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Pig. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NNT recombinant protein (Position: C44-A350).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Monkey, Rat, Pig
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hasselmann, C., Deladoey, J., Vuissoz, J.-M., Patry, L., Alirezaie, N., Schwartzentruber, J., FORGE Canada Consortium, Deal, C. L., Van Vliet, G., Majewski, J., Samuels, M. E. Expanding the phenotypic spectrum of nicotinamide nucleotide transhydrogenase (NNT) mutations and using whole exome sequencing to discover potential disease modifiers. J. Genomes Exomes 2: 19-30, 2013. 2. Meimaridou, E., Kowalczyk, J., Guasti, L., Hughes, C. R., Wagner, F., Frommolt, P., Nurnberg, P., Mann, N. P., Banerjee, R., Saka, H. N., Chapple, J. P., King, P. J., Clark, A. J. L., Metherell, L. A. Mutations in NNT encoding nicotinamide nucleotide transhydrogenase cause familial glucocorticoid deficiency. Nature Genet. 44: 740-742, 2012. 3. Roucher-Boulez, F., Mallet-Motak, D., Samara-Boustani, D., Jilani, H., Ladjouze, A., Souchon, P.-F., Simon, D., Nivot, S., Heinrichs, C., Ronze, M., Bertagna, X., Groisne, L., Leheup, B., Naud-Saudreau, C., Blondin, G., Lefevre, C., Lemarchand, L., Morel, Y. NNT mutations: a cause of primary adrenal insufficiency, oxidative stress and extra-adrenal defects. Europ. J. Endocr. 175: 73-84, 2016.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NNT

Target: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Andere Bezeichnung: NNT

Hintergrund:

Synonyms: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, GCK

Tissue Specificity: Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.

Background: This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD (H) and NADP (+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.

Molekulargewicht: 114 kDa

Gen-ID: 23530

UniProt: Q13423

Pathways: Proton Transport