Glucuronidase beta Antikörper (AA 416-651)

Produktnummer ABIN7601685

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Glucuronidase beta in WB, ELISA, ICC, FACS und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Glucuronidase beta Antikörper (AA 416-651) (ABIN7601685)

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucuronidase beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-Glucuronidase beta Antikörper

Bindungsspezifität: AA 416-651

Verwendungszweck: Anti-beta glucuronidase/GUSB Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-beta glucuronidase/GUSB Antibody Picoband® (ABIN7601685). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human beta glucuronidase/GUSB recombinant protein (Position: Q416-T651).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kreamer, B. L., Siegel, F. L., Gourley, G. R. A novel inhibitor of beta-glucuronidase: L-aspartic acid. Pediat. Res. 50: 460-466, 2001. 2. Miller, R. D., Hoffmann, J. W., Powell, P. P., Kyle, J. W., Shipley, J. M., Bachinsky, D. R., Sly, W. S. Cloning and characterization of the human beta-glucuronidase gene. Genomics 7: 280-283, 1990. 3. Tomatsu, S., Orii, K. O., Vogler, C., Grubb, J. H., Snella, E. M., Gutierrez, M. A., Dieter, T., Sukegawa, K., Orii, T., Kondo, N., Sly, W. S. Missense models [Gus(tm(E536A)Sly), Gus(tm(E536Q)Sly), and Gus(tm(L175F)Sly)] of murine mucopolysaccharidosis type VII produced by targeted mutagenesis. Proc. Nat. Acad. Sci. 99: 14982-14987, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Glucuronidase beta

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Andere Bezeichnung: GUSB

Hintergrund:

Synonyms: Beta-glucuronidase, Beta-G1, GUSB

Tissue Specificity: Broadly expressed with higher expression in heart.

Background: Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.

Molekulargewicht: 82 kDa

Gen-ID: 2990

UniProt: P08236

Pathways: Glycosaminoglycan Metabolic Process