Glucuronidase beta Antikörper

Produktnummer ABIN458831

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-Glucuronidase beta Antikörper wird verwendet zum Nachweis von Glucuronidase beta in Proben von E. coli. Er wurde validiert für WB, ELISA, DB und IFA.

Kurzübersicht für Glucuronidase beta Antikörper (ABIN458831)

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucuronidase beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Produktdetails anti-Glucuronidase beta Antikörper

Verwendungszweck: Rabbit anti Escherichia coli Glucuronidase

Spezifität: IgG fraction of polyclonal Rabbit antiSerum to beta-glucuronidase from Escherichia coli.

Kreuzreaktivität (Details): Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: beta-Glucuronidase is isolated and purified from Escherichia coli. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Referenzen für anti-Glucuronidase beta Antikörper (ABIN458831)

Mickoleit, Rosenfeldt, Toro-Nahuelpan, Schaffer, Schenk, Plitzko, Schüler: "High-Yield Production, Characterization, and Functionalization of Recombinant Magnetosomes in the Synthetic Bacterium Rhodospirillum rubrum "magneticum"." in: Advanced biology, pp. e2101017, (2021) (PubMed).

Mickoleit, Lanzloth, Schüler: "A Versatile Toolkit for Controllable and Highly Selective Multifunctionalization of Bacterial Magnetic Nanoparticles." in: Small (Weinheim an der Bergstrasse, Germany), pp. e1906922, (2020) (PubMed).

Details zu Glucuronidase beta

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Andere Bezeichnung: beta-glucuronidase

UniProt: P05804

Pathways: Glycosaminoglycan Metabolic Process