SPRED2 Antikörper (AA 40-298)

Produktnummer ABIN7601643

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SPRED2 in WB, ELISA, ICC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für SPRED2 Antikörper (AA 40-298) (ABIN7601643)

Target: SPRED2 (Sprouty-Related, EVH1 Domain Containing 2 (SPRED2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPRED2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-SPRED2 Antikörper

Bindungsspezifität: AA 40-298

Verwendungszweck: Anti-SPRED2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-SPRED2 Antibody Picoband® (ABIN7601643). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SPRED2 recombinant protein (Position: R40-R298).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bundschu, K., Knobeloch, K.-P., Ullrich, M., Schinke, T., Amling, M., Engelhardt, C. M., Renne, T., Walter, U., Schuh, K. Gene disruption of Spred-2 causes dwarfism. J. Biol. Chem. 280: 28572-28580, 2005. 2. Kato, R., Nonami, A., Taketomi, T., Wakioka, T., Kuroiwa, A., Matsuda, Y., Yoshimura, A. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun. 302: 767-772, 2003. 3. Motta, M., Fasano, G., Gredy, S., Brinkmann, J., Bonnard, A. A., Simsek-Kiper, P. O., Gulec, E. Y., Essaddam, L., Utine, G. E., Guarnetti Prandi, I., Venditti, M., Pantaleoni, F., and 21 others. SPRED2 loss-of-function causes a recessive Noonan syndrome-like phenotype. Am. J. Hum. Genet. 108: 2112-2129, 2021.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu SPRED2

Target: SPRED2 (Sprouty-Related, EVH1 Domain Containing 2 (SPRED2))

Andere Bezeichnung: SPRED2

Hintergrund:

Synonyms: Rho-related GTP-binding protein Rho6,Rho family GTPase 1,Rnd1,RND1,RHO6,

Tissue Specificity: Mostly expressed in brain and liver.

Background: Sprouty-related, EVH1 domain-containing protein 2 is a protein that in humans is encoded by the SPRED2 gene. SPRED2 is a member of the Sprouty (see SPRY1, MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade.

Molekulargewicht: 53 kDa

Gen-ID: 200734

UniProt: Q7Z698