HECTD1 Antikörper (AA 39-566)

Produktnummer ABIN7601584

Der Kaninchen Polyklonal Anti-HECTD1-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, HECTD1 in Proben von Human zu detektieren.

Kurzübersicht für HECTD1 Antikörper (AA 39-566) (ABIN7601584)

Target: HECTD1 (HECT Domain Containing 1 (HECTD1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HECTD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-HECTD1 Antikörper

Bindungsspezifität: AA 39-566

Verwendungszweck: Anti-HECTD1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-HECTD1 Antibody Picoband® (ABIN7601584). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human HECTD1 recombinant protein (Position: D39-S566).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aleidi, S. M., Yang, A., Sharpe, L. J., Rao, G., Cochran, B. J., Rye, K.-A., Kockx, M., Brown, A. J., Gelissen, I. C. The E3 ubiquitin ligase, HECTD1, is involved in ABCA1-mediated cholesterol export from macrophages. Biochim. Biophys. Acta Molec. Cell Biol. Lipids 1863: 359-368, 2018. 2. D'Alonzo, D., Emch, F. H., Shen, X., Bruder, E., De Geyter, C., Zhang, H. Hectd1 is essential for embryogenesis in mice. Gene Expr. Patterns 34: 119064, 2019. Note: Electronic Article. 3. Gross, M. B. Personal Communication. Baltimore, Md. 10/29/2019.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu HECTD1

Target: HECTD1 (HECT Domain Containing 1 (HECTD1))

Andere Bezeichnung: HECTD1

Hintergrund:

Synonyms: Kallistatin, Kallikrein inhibitor, Peptidase inhibitor 4, PI-4, Serpin A4, SERPINA4, KST, PI4

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then ly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This protein is specific to HECTD1.

Molekulargewicht: 290 kDa

Gen-ID: 25831

Pathways: Tube Formation, SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion