Chromogranin A Antikörper (AA 378-425)

Produktnummer ABIN7601533

Der Kaninchen Polyklonal Anti-Chromogranin A-Antikörper wurde für IHC, FACS, WB und ELISA validiert. Er ist geeignet, Chromogranin A in Proben von Ratte und Maus zu detektieren.

Kurzübersicht für Chromogranin A Antikörper (AA 378-425) (ABIN7601533)

Target: Chromogranin A (CHGA)

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Chromogranin A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), ELISA

Produktdetails anti-Chromogranin A Antikörper

Bindungsspezifität: AA 378-425

Verwendungszweck: Anti-Chromogranin A/Chga Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Chromogranin A/Chga Antibody Picoband® (ABIN7601533). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Chga recombinant protein (Position: D378-K425).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Helman LJ, Ahn TG, Levine MA, Allison A, Cohen PS, Cooper MJ, Cohn DV, Israel MA (August 1988). "Molecular cloning and primary structure of human chromogranin A (secretory protein I) cDNA". J. Biol. Chem. 263 (23): 11559-63. 2. Cotesta D, Caliumi C, Al111 P, Petramala L, Reale MG, Masciangelo R, Signore A, Cianci R, D'Erasmo E, Letizia C (2005). "High plasma levels of human chromogranin A and adrenomedullin in patients with pheochromocytoma". Tumori 91 (1): 53-8.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Chromogranin A

Target: Chromogranin A (CHGA)

Andere Bezeichnung: Chga

Hintergrund:

Synonyms: Kit ligand, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, Soluble KIT ligand, sKITLG, KITLG, MGF, SCF,

Background: CHGA, also known as CGA or chromogranin A, is an acidic protein costored and coreleased with catecholamines from storage granules in the adrenal medulla. CHGA is a member of the granin family of neuroendocrine secretory protein, It is located in secretory vesicles of neurons and endocrine cells. In humans, CHGA protein is encoded by the CHGA gene, and it is mapped to 14q32.12. CHGA gene product is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine).

Molekulargewicht: 80 kDa

Gen-ID: 12652

UniProt: P26339

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling