CD73 Antikörper (AA 337-551)

Produktnummer ABIN7601367

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CD73 in WB, IHC, FACS und ELISA. Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für CD73 Antikörper (AA 337-551) (ABIN7601367)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD73 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Produktdetails anti-CD73 Antikörper

Bindungsspezifität: AA 337-551

Verwendungszweck: Anti-CD73/Nt5e Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CD73/Nt5e Antibody Picoband® (ABIN7601367). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD73/Nt5e recombinant protein (Position: S337-S551).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Boyle, J. M., Hey, Y., Fox, M. Human ecto-5-prime nucleotidase maps to chromosome 6q14-q21. (Abstract) Cytogenet. Cell Genet. 51: 968 only, 1989. 2. Castrop, H., Huang, Y., Hashimoto, S., Mizel, D., Hansen, P., Theilig, F., Bachmann, S., Deng, C., Briggs, J., Schnermann, J. Impairment of tubuloglomerular feedback regulation of GFR in ecto-5-prime-nucleotidase/CD73-deficient mice. J. Clin. Invest. 114: 634-642, 2004. 3. St. Hilaire, C., Ziegler, S. G., Markello, T. C., Brusco, A., Groden, C., Gill, F., Carlson-Donohoe, H., Lederman, R. J., Chen, M. Y., Yang, D., Siegenthaler, M. P., Arduino, C., and 9 others. NT5E mutations and arterial calcifications. New Eng. J. Med. 364: 432-442, 2011.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Andere Bezeichnung: Nt5e

Hintergrund:

Synonyms: Carnitine O-palmitoyltransferase 2, mitochondrial, Carnitine palmitoyltransferase II, CPT II, CPT2, CPT1

Tissue Specificity: Expressed mainly in adipose tissues.

Background: The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 63 kDa

Gen-ID: 23959

UniProt: Q61503

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process