Glypican 3 Antikörper (AA 32-535)

Produktnummer ABIN7601314

Der Kaninchen Polyklonal Anti-Glypican 3-Antikörper wurde für FACS, WB und ELISA validiert. Er ist geeignet, Glypican 3 in Proben von Human zu detektieren.

Kurzübersicht für Glypican 3 Antikörper (AA 32-535) (ABIN7601314)

Target: Glypican 3 (GPC3)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glypican 3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Produktdetails anti-Glypican 3 Antikörper

Bindungsspezifität: AA 32-535

Verwendungszweck: Anti-Glypican 3/GPC3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Glypican 3/GPC3 Antibody Picoband® (ABIN7601314). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Glypican 3/GPC3 recombinant protein (Position: D32-A535).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Capurro, M. I., Xu, P., Shi, W., Li, F., Jia, A., Filmus, J. Glypican-3 inhibits Hedgehog signaling during development by competing with Patched for Hedgehog binding. Dev. Cell 14: 700-711, 2008. 2. Filmus, J., Capurro, M., Rast, J. Glypicans Genome Biol. 9: 224, 2008. Note: Electronic Article. 3. Filmus, J., Church, J. G., Buick, R. N. Isolation of a cDNA corresponding to a developmentally regulated transcript in rat intestine. Molec. Cell Biol. 8: 4243-4249, 1988.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Glypican 3

Target: Glypican 3 (GPC3)

Andere Bezeichnung: GPC3

Hintergrund:

Synonyms: Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Glypican-3 alpha subunit, Glypican-3 beta subunit, GPC3, OCI5

Tissue Specificity: Highly expressed in lung, liver and kidney.

Background: Glypican-3 is a protein that, in humans, is encoded by the GPC3 gene. It is mapped to Xq26.2. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 66 kDa

Gen-ID: 2719

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process