CD82 Antikörper (AA 32-266)

Produktnummer ABIN7601301

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CD82 in ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für CD82 Antikörper (AA 32-266) (ABIN7601301)

Target: CD82

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD82 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-CD82 Antikörper

Bindungsspezifität: AA 32-266

Verwendungszweck: Anti-CD82 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CD82 Antibody Picoband® (ABIN7601301). Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD82 recombinant protein (Position: V32-Y266).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Dong, J.-T., Lamb, P. W., Rinker-Schaeffer, C. W., Vukanovic, J., Ichikawa, T., Isaacs, J. T., Barrett, J. C. : KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science 268: 884-886, 1995. 2. Mashimo, T., Watabe, M., Hirota, S., Hosobe, S., Miura, K., Tegtmeyer, P. J., Rinker-Shaeffer, C. W., Watabe, K. : The expression of the KAI1 gene, a tumor metastasis suppressor, is ly activated by p53. Proc. Nat. Acad. Sci. 95: 11307-11311, 1998. 3. Miyazaki, T., Kato, H., Shitara, Y., Yoshikawa, M., Tajima, K., Masuda, N., Shouji, H., Tsukada, K., Nakajima, T., Kuwano, H. : Mutation and expression of the metastasis suppressor gene KAI1 in esophageal squamous cell carcinoma. Cancer 89: 955-962, 2000. 4. Jee, B. K., Park, K. M., Surendran, S., Lee, W. K., Han, C. W., Kim, Y. S., Lim, Y. : KAI1/CD82 suppresses tumor invasion by MMP9 inactivation via TIMP1 up-regulation in the H1299 human lung carcinoma cell line. Biochem. Biophys. Res. Commun. 342: 655-661, 2006.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CD82

Target: CD82

Andere Bezeichnung: Cd82

Hintergrund:

Synonyms: Tumor necrosis factor receptor superfamily member 14,Herpes virus entry mediator A,Herpesvirus entry mediator A,HveA,Tumor necrosis factor receptor-like 2,TR2,CD270,TNFRSF14,HVEA, HVEM,UNQ329/PRO509,

Tissue Specificity: Widely expressed, with the highest expression in lung, spleen and thymus.

Background: CD82 (Cluster of Differentiation 82), also named KAI1, is a human protein encoded by the CD82 gene. The gene is mapped to 11p11.2. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The expression of CD82 protein appears to be correlated with lymph node metastasis in esophageal squamous cell carcinoma (ESCC). And the CD82 overexpression can suppress tumor invasiveness and metastatic potential by inducing MMP9 inactivation via upregulation of TIMP1.

Molekulargewicht: 80-85 kDa

Gen-ID: 12521

UniProt: P40237

Pathways: p53 Signalweg