CD226 Antikörper (AA 31-333)

Produktnummer ABIN7601264

Dieses Anti-CD226-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CD226 in WB, FACS, ELISA und IHC. Geeignet für Maus und Ratte.

Kurzübersicht für CD226 Antikörper (AA 31-333) (ABIN7601264)

Target: CD226

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD226 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-CD226 Antikörper

Bindungsspezifität: AA 31-333

Verwendungszweck: Anti-CD226 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti- Antibody Picoband® (ABIN7601264). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD226 recombinant protein (Position: E31-L333).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Bottino, C., Castriconi, R., Pende, D., Rivera, P., Nanni, M., Carnemolla, B., Cantoni, C., Grassi, J., Marcenaro, S., Reymond, N., Vitale, M., Moretta, L., Lopez, M., Moretta, A. Identification of PVR (CD155) and nectin-2 (CD112) as cell surface ligands for the human DNAM-1 (CD226) activating molecule. J. Exp. Med. 198: 557-567, 2003. 2. Iguchi-Manaka, A., Kai, H., Yamashita, Y., Shibata, K., Tahara-Hanaoka, S., Honda, S., Yasui, T., Kikutani, H., Shibuya, K., Shibuya, A. Accelerated tumor growth in mice deficient in DNAM-1 receptor. J. Exp. Med. 205: 2959-2964, 2008. 3. Liu, J., Qian, X., Chen, Z., Xu, X., Gao, F., Zhang, S., Zhang, R., Qi, J., Gao, G. F., Yan, J. Crystal structure of cell adhesion molecule nectin-2/CD112 and its binding to immune receptor DNAM-1/CD226. J Immun. 188: 5511-5520, 2012.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CD226

Target: CD226

Andere Bezeichnung: Cd226

Hintergrund:

Synonyms: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform,PI3-kinase subunit beta,PI3K-beta,PI3Kbeta,PtdIns-3-kinase subunit beta,2.7.1.153,Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta,PtdIns-3-kinase subunit p110-beta,p110beta,PIK3CB,PIK3C1,

Tissue Specificity: Expressed ubiquitously.

Background: DNAM 1, also known as CD226, is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes. This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.

Molekulargewicht: 70 kDa

Gen-ID: 225825

UniProt: Q8K4F0

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints