AKR7A2 Antikörper (AA 294-359)

Produktnummer ABIN7601157

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AKR7A2 in WB, IHC, ELISA und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für AKR7A2 Antikörper (AA 294-359) (ABIN7601157)

Target: AKR7A2 (Aflatoxin Beta1 Aldehyde Reductase (AKR7A2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKR7A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Produktdetails anti-AKR7A2 Antikörper

Bindungsspezifität: AA 294-359

Verwendungszweck: Anti-AKR7A2 Picoband® Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-AKR7A2 Picoband® Antibody (ABIN7601157). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human AKR7A2 recombinant protein (Position: A294-R359).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ireland, L. S., Harrison, D. J., Neal, G. E., Hayes, J. D. Molecular cloning, expression and catalytic activity of a human AKR7 member of the aldo-keto reductase superfamily: evidence that the major 2-carboxybenzaldehyde reductase from human liver is a homologue of rat aflatoxin B(1)-aldehyde reductase. Biochem. J. 332: 21-34, 1998. 2. Kelly, V. P., Sherratt, P. J., Crouch, D. H., Hayes, J. D. Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of reductase 7 family proteins. Biochem. J. 366: 847-861, 2002. 3. Praml, C., Savelyeva, L., Schwab, M. Aflatoxin B1 aldehyde reductase (AFAR) genes cluster at 1p35-1p36.1 in a region frequently altered in human tumour cells. Oncogene 22: 4765-4773, 2003.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu AKR7A2

Target: AKR7A2 (Aflatoxin Beta1 Aldehyde Reductase (AKR7A2))

Andere Bezeichnung: AKR7A2

Hintergrund:

Synonyms: Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, AFB1-AR 1, Aldoketoreductase 7, Succinic semialdehyde reductase, SSA reductase, AKR7A2, AFAR, AFAR1, AKR7

Tissue Specificity: Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.

Background: Aflatoxin B1 aldehyde reductase member 2 is an enzyme that in humans is encoded by the AKR7A2 gene. It is mapped to 1p36.13. The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 36 kDa

Gen-ID: 8574

UniProt: O43488