Caspase 10 Antikörper (AA 271-482)

Produktnummer ABIN7601039

Dieses Anti-Caspase 10-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Caspase 10 in WB, ELISA und FACS. Geeignet für Human.

Kurzübersicht für Caspase 10 Antikörper (AA 271-482) (ABIN7601039)

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-Caspase 10 Antikörper

Bindungsspezifität: AA 271-482

Verwendungszweck: Anti-Caspase-10/CASP10 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Caspase-10/CASP10 Antibody Picoband® (ABIN7601039). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Caspase-10/CASP10 recombinant protein (Position: K271-E482).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Lee, H.-J., Pyo, J.-O., Oh, Y., Kim, H.-J., Hong, S., Jeon, Y.-J., Kim, H., Cho, D.-H., Woo, H.-N., Song, S., Nam, J.-H., Kim, H. J., Kim, K.-S., Jung, Y.-K.AK2 activates a novel apoptotic pathway through formation of a complex with FADD and caspase-10.Nature Cell Biol. 9: 1303-1310, 2007. 2. Park, W. S., Lee, J. H., Shin, M. S., Park, J. Y., Kim, H. S., Lee, J. H., Kim, Y. S., Lee, S. N., Xiao, W., Park, C. H., Lee, S. H., Yoo, N. J., Lee, J. Y.Inactivating mutations of the caspase-10 gene in gastric cancer.Oncogene 21: 2919-2925, 2002. 3. Wang, J., Zheng, L., Lobito, A., Chan, F. K., Dale, J., Sneller, M., Yao, X., Puck, J. M., Straus, S. E., Lenardo, M. J.Inherited human caspase 10 mutations underlie defective lymphocyte and dendritic cell apoptosis in autoimmune lymphoproliferative syndrome type II.Cell 98: 47-58, 1999.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu Caspase 10

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Andere Bezeichnung: CASP10

Hintergrund:

Synonyms: Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD235a, GYPA, GPA

Tissue Specificity: Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.

Background: Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. The Caspase 10 gene contains 11 exons and spans about 48 kb. This gene is mapped to 2q33.1. It is transcribed in the centromere-to-telomere ion.This gene encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Molekulargewicht: 59 kDa

Gen-ID: 843

UniProt: Q92851

Pathways: Apoptose, Caspase Kaskade in der Apoptose