EZH1 Antikörper (AA 27-485)

Produktnummer ABIN7601019

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EZH1 in WB, ELISA, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für EZH1 Antikörper (AA 27-485) (ABIN7601019)

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EZH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-EZH1 Antikörper

Bindungsspezifität: AA 27-485

Verwendungszweck: Anti-EZH1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-EZH1 Antibody Picoband® (ABIN7601019). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human EZH1 recombinant protein (Position: R27-E485).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Abel, K. J., Brody, L. C., Valdes, J. M., Erdos, M. R., McKinley, D. R., Castilla, L. H., Merajver, S. D., Couch, F. J., Friedman, L. S., Ostermeyer, E. A., Lynch, E. D., King, M.-C., Welcsh, P. L., Osborne-Lawrence, S., Spillman, M., Bowcock, A. M., Collins, F. S., Weber, B. L. Characterization of EZH1, a human homolog of Drosophila enhancer of zeste near BRCA1. Genomics 37: 161-171, 1996. 2. Laible, G., Haynes, A. R., Lebersorger, A., O'Carroll, D., Mattei, M.-G., Denny, P., Brown, S. D. M., Jenuwein, T. The murine polycomb-group genes Ezh1 and Ezh2 map close to Hox gene clusters on mouse chromosomes 11 and 6. Mammalian Genome 10: 311-314, 1999. 3. Maier, V. K., Feeney, C. M., Taylor, J. E., Creech, A. L., Qiao, J. W., Szanto, A., Das, P. P., Chevrier, N., Cifuentes-Rojas, C., Orkin, S. H., Carr, S. A., Jaffe, J. D., Mertins, P., Lee, J. T. Functional proteomic analysis of repressive histone methyltransferase complexes reveals ZNF518B as a G9A regulator. Molec. Cell. Proteomics 14: 1435-1446, 2015.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu EZH1

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Andere Bezeichnung: EZH1

Hintergrund:

Synonyms: Heat shock protein beta-8,HspB8,Alpha-crystallin C chain,E2-induced gene 1 protein,Protein kinase H11,Small stress protein-like protein HSP22,HSPB8,CRYAC, E2IG1, HSP22,PP1629,

Tissue Specificity: Predominantly expressed in skeletal muscle and heart. .

Background: Histone-lysine N-methyltransferase EZH1 is an enzyme that in humans is encoded by the EZH1 gene. EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity.

Molekulargewicht: 110 kDa, 60 kDa

Gen-ID: 2145

UniProt: Q92800