Liver Arginase Antikörper (AA 25-183)

Produktnummer ABIN7600888

Der Maus Monoklonal Anti-Liver Arginase-Antikörper wurde für WB und FACS validiert. Er ist geeignet, Liver Arginase in Proben von Human, Ratte, Maus und Affe zu detektieren.

Kurzübersicht für Liver Arginase Antikörper (AA 25-183) (ABIN7600888)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reaktivität: Human, Ratte, Maus, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Liver Arginase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: 2B12

Produktdetails anti-Liver Arginase Antikörper

Bindungsspezifität: AA 25-183

Verwendungszweck: Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) (ABIN7600888). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3μ/1x10⁶ cells, Human1. Cardoso, M. L., Martins, E., Vasconcelos, R., Vilarinho, L., Rocha, J. Identification of a novel R21X mutation in the liver-type arginase gene (ARG1) in four Portuguese patients with argininemia. Hum. Mutat. 14: 355-356, 1999. 2. Grody, W. W., Dodson, A., Klein, D., Kern, R. M., Bassand, P., Cederbaum, S. D. Molecular genetic study of human arginase deficiency. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A191 only, 1989. 3. Haraguchi, Y., Takiguchi, M., Amaya, Y., Kawamoto, S., Matsuda, I., Mori, M. Molecular cloning and nucleotide sequence of cDNA for human liver arginase. Proc. Nat. Acad. Sci. 84: 412-415, 1987.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Andere Bezeichnung: ARG1

Hintergrund:

Synonyms: Putative Polycomb group protein ASXL1, Additional sex combs-like protein 1, ASXL1, KIAA0978

Tissue Specificity: Widely expressed at low level. Expressed in heart,brain,skeletal muscle,placenta,pancreas,spleen,prostate,mall intestine,colon,peripheral blood,leukocytes,bone marrow and fetal liver.Highly expressed in testes.

Background: ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98 % of the arginase activity in liver but is also present in red cells. Using a rat liverARG1cDNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90 % of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50 % of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 35 kDa

Gen-ID: 383

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin